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takara reverse transcription kit  (TaKaRa)


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    Structured Review

    TaKaRa takara reverse transcription kit
    Takara Reverse Transcription Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 13586 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/takara+reverse+transcription+kit/PCR+Amplification+Kit/pmc13144552-105-8-8
    Average 96 stars, based on 13586 article reviews
    takara reverse transcription kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: QiangGuYin-containing serum improves osteogenic differentiation via regulating osteoclast-derived exosomal miR-27b-3p.
    Article Snippet: 1 Department of Orthopedics, The Second Affiliated Hospital of Zhejiang Chinese Medical University (Xinhua Hospital of Zhejiang Province), 318 Chaowang Road, Gongshu District, Hangzhou 310000, Zhejiang, People’s Republic of China 2 The Second School of Clinical Medicine, Zhejiang Chinese Medical University, 548 Binwen Road, Puyan Street, Binjiang District, Hangzhou 310000, Zhejiang, People’s Republic of China Abstract Osteoclast-derived exosomes play a critical role in suppressing osteogenic differentiation, contributing to postmenopausal osteoporosis (PMOP).. QiangGuYin (QGY), a traditional Chinese herbal formula, has demonstrated efficacy in alleviating PMOP progression.. This study aimed to elucidate the mechanism by which QGY modulates osteoclast-derived exosomemediated inhibition of osteogenesis.

    Article Title: Interleukin-7 induces EMT to promote tumor growth and metastasis in NSCLC via Notch1/TGF-β pathway.
    Article Snippet: Total RNA was isolated from cells and tissues using TRIZOL (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. .. According to the Takara Reverse Transcription Kit (Takara, Tokyo, Japan, RR047A) procedure cDNA was obtained by reverse transcription of the RNA under the following conditions: 37°C for 15 min; 85°C for 5 s. The subsequent fluorescence quantitative amplification was carried out under the following conditions: 95°C for 30 s, followed by 40 cycles of 95°C for 5 s, 60°C for 34 s, 95°C for 15 min, 60°C for 1 min and 95°C for 15 s to obtain the amplification curve. ..

    Article Title: Oncogenic and immunological roles of LAMP5 across cancers and its potential utility in bladder cancer.
    Article Snippet: Zhenni Lin and Kun Zhao contributed equally to this work.. Abstract Lysosomal associated membrane protein family member 5 (LAMP5), a recently identified member of the LAMP family, has been associated with poor prognosis in multiple cancer types; however, its precise oncogenic mechanisms remain unclear.. This study systematically investigated the oncogenic and immunological functions of LAMP5 using multiple datasets.

    Article Title: Engineering a mouse-adapted SADS-CoV and establishing a neonatal mouse model to study its infection
    Article Snippet: .. After RNA was extracted from tissue samples, reverse transcription was performed according to the instructions of the Takara reverse transcription kit (RR036A). .. The primers used were designed on the basis of the conserved sequences of the SADS-CoV N gene and the MHV-A59 S gene , and reverse transcription PCR was performed using DNA polymerase (CWBIO, China).

    Article Title: Apoptotic metabolites synthesize and inherit unique de novo L2a/L2b RNAs to prevent virus infection
    Article Snippet: .. 500 ng RNA from each group was used in 10 μL reverse transcription system, which was configured with Takara reverse transcription kit (#RR037A, Takara). .. Then the cDNA was amplified by Mastercycler X50a (Eppendorf AG, Germany).

    Article Title: A novel CLDN6 nanobody-based bispecific T-cell engager delivered by self-amplifying RNA platform exhibits potent antitumor efficacy
    Article Snippet: .. Total RNA was extracted from peripheral blood lymphocytes using the Promega RNA extraction kit, followed immediately by cDNA synthesis with a TaKaRa reverse transcription kit (cat. no. RR036A). ..

    Article Title: Integrated Network Toxicology and Transcriptomics Reveal Molecular Mechanisms of Cadmium-Exposed Liver Injury in Swine.
    Article Snippet: Hub nodes were defined as those with degree, betweenness centrality, and closeness centrality above the network median, and key modules were extracted using the MCODE plugin (Cytoscape App Store; Bader Lab, University of Toronto, Toronto, ON, Canada) [20]. .. Total RNA from liver tissues was extracted with TRIzol (Thermo Fisher Scientific, Waltham, MA, USA) and reverse-transcribed to cDNA using the TaKaRa Reverse Transcription Kit (RR036A; Takara Bio Inc., Shiga, Japan). qPCR was performed on a LineGene 9600 Plus system (FQD-96A; Bioer Technology Co., Ltd., Hangzhou, China) using Talent qPCR PreMix (SYBR Green) (A25742, TIANGEN, Beijing, China). ..

    Article Title: Pachymic Acid Alleviates Non‐Alcoholic Fatty Liver Disease via FGF21 ‐Mediated Inhibition of the p38 MAPK Pathway
    Article Snippet: .. RNA was then reverse‐transcribed into cDNA with a Takara reverse transcription kit, followed by amplification and quantification by qRT‐PCR. ..

    Real-time Polymerase Chain Reaction:

    Article Title: QiangGuYin-containing serum improves osteogenic differentiation via regulating osteoclast-derived exosomal miR-27b-3p.
    Article Snippet: 1 Department of Orthopedics, The Second Affiliated Hospital of Zhejiang Chinese Medical University (Xinhua Hospital of Zhejiang Province), 318 Chaowang Road, Gongshu District, Hangzhou 310000, Zhejiang, People’s Republic of China 2 The Second School of Clinical Medicine, Zhejiang Chinese Medical University, 548 Binwen Road, Puyan Street, Binjiang District, Hangzhou 310000, Zhejiang, People’s Republic of China Abstract Osteoclast-derived exosomes play a critical role in suppressing osteogenic differentiation, contributing to postmenopausal osteoporosis (PMOP).. QiangGuYin (QGY), a traditional Chinese herbal formula, has demonstrated efficacy in alleviating PMOP progression.. This study aimed to elucidate the mechanism by which QGY modulates osteoclast-derived exosomemediated inhibition of osteogenesis.

    Article Title: Integrated Network Toxicology and Transcriptomics Reveal Molecular Mechanisms of Cadmium-Exposed Liver Injury in Swine.
    Article Snippet: Hub nodes were defined as those with degree, betweenness centrality, and closeness centrality above the network median, and key modules were extracted using the MCODE plugin (Cytoscape App Store; Bader Lab, University of Toronto, Toronto, ON, Canada) [20]. .. Total RNA from liver tissues was extracted with TRIzol (Thermo Fisher Scientific, Waltham, MA, USA) and reverse-transcribed to cDNA using the TaKaRa Reverse Transcription Kit (RR036A; Takara Bio Inc., Shiga, Japan). qPCR was performed on a LineGene 9600 Plus system (FQD-96A; Bioer Technology Co., Ltd., Hangzhou, China) using Talent qPCR PreMix (SYBR Green) (A25742, TIANGEN, Beijing, China). ..

    Fluorescence:

    Article Title: Interleukin-7 induces EMT to promote tumor growth and metastasis in NSCLC via Notch1/TGF-β pathway.
    Article Snippet: Total RNA was isolated from cells and tissues using TRIZOL (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. .. According to the Takara Reverse Transcription Kit (Takara, Tokyo, Japan, RR047A) procedure cDNA was obtained by reverse transcription of the RNA under the following conditions: 37°C for 15 min; 85°C for 5 s. The subsequent fluorescence quantitative amplification was carried out under the following conditions: 95°C for 30 s, followed by 40 cycles of 95°C for 5 s, 60°C for 34 s, 95°C for 15 min, 60°C for 1 min and 95°C for 15 s to obtain the amplification curve. ..

    Amplification:

    Article Title: Interleukin-7 induces EMT to promote tumor growth and metastasis in NSCLC via Notch1/TGF-β pathway.
    Article Snippet: Total RNA was isolated from cells and tissues using TRIZOL (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. .. According to the Takara Reverse Transcription Kit (Takara, Tokyo, Japan, RR047A) procedure cDNA was obtained by reverse transcription of the RNA under the following conditions: 37°C for 15 min; 85°C for 5 s. The subsequent fluorescence quantitative amplification was carried out under the following conditions: 95°C for 30 s, followed by 40 cycles of 95°C for 5 s, 60°C for 34 s, 95°C for 15 min, 60°C for 1 min and 95°C for 15 s to obtain the amplification curve. ..

    Article Title: Pachymic Acid Alleviates Non‐Alcoholic Fatty Liver Disease via FGF21 ‐Mediated Inhibition of the p38 MAPK Pathway
    Article Snippet: .. RNA was then reverse‐transcribed into cDNA with a Takara reverse transcription kit, followed by amplification and quantification by qRT‐PCR. ..

    Synthesized:

    Article Title: Oncogenic and immunological roles of LAMP5 across cancers and its potential utility in bladder cancer.
    Article Snippet: Zhenni Lin and Kun Zhao contributed equally to this work.. Abstract Lysosomal associated membrane protein family member 5 (LAMP5), a recently identified member of the LAMP family, has been associated with poor prognosis in multiple cancer types; however, its precise oncogenic mechanisms remain unclear.. This study systematically investigated the oncogenic and immunological functions of LAMP5 using multiple datasets.

    RNA Extraction:

    Article Title: A novel CLDN6 nanobody-based bispecific T-cell engager delivered by self-amplifying RNA platform exhibits potent antitumor efficacy
    Article Snippet: .. Total RNA was extracted from peripheral blood lymphocytes using the Promega RNA extraction kit, followed immediately by cDNA synthesis with a TaKaRa reverse transcription kit (cat. no. RR036A). ..

    cDNA Synthesis:

    Article Title: A novel CLDN6 nanobody-based bispecific T-cell engager delivered by self-amplifying RNA platform exhibits potent antitumor efficacy
    Article Snippet: .. Total RNA was extracted from peripheral blood lymphocytes using the Promega RNA extraction kit, followed immediately by cDNA synthesis with a TaKaRa reverse transcription kit (cat. no. RR036A). ..

    SYBR Green Assay:

    Article Title: Integrated Network Toxicology and Transcriptomics Reveal Molecular Mechanisms of Cadmium-Exposed Liver Injury in Swine.
    Article Snippet: Hub nodes were defined as those with degree, betweenness centrality, and closeness centrality above the network median, and key modules were extracted using the MCODE plugin (Cytoscape App Store; Bader Lab, University of Toronto, Toronto, ON, Canada) [20]. .. Total RNA from liver tissues was extracted with TRIzol (Thermo Fisher Scientific, Waltham, MA, USA) and reverse-transcribed to cDNA using the TaKaRa Reverse Transcription Kit (RR036A; Takara Bio Inc., Shiga, Japan). qPCR was performed on a LineGene 9600 Plus system (FQD-96A; Bioer Technology Co., Ltd., Hangzhou, China) using Talent qPCR PreMix (SYBR Green) (A25742, TIANGEN, Beijing, China). ..



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